• Selective Enrichment to Obtain an Indigenous Microbial Consortium Degrading Recalcitrant TPHs(total petroleum hydrocarbons) from Petroleum-contaminated Soil in Kuwait
  • Jinho Ha1·Seonghoon Kim2·Hyunsoo Lim1·Woosik Jung1·Dajung Kim1·Keumyoung Lee3·Joonhong Park1*

  • 1School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Korea
    2Hankuk Engineering Consultant Co., Ltd, Gyeonggi-Do 14056, Korea
    3H-Plus Eco Ltd, Seoul 05610, Korea

  • 쿠웨이트 원유오염 토양 내 잔류 난분해성 유기물 분해능 지닌 토착 미생물 배양체 획득을 위한 선택적 계대배양 실험 연구
  • 하진호1·김성훈2·임현수1·정우식1·김다정1·이금영3·박준홍1*

  • 1연세대학교 사회환경시스템공학부

  • This article is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


In this work, an indigenous microbial consortium was obtained by selectively cultivating microbes using a long-aged petroleum-contaminated soil (Kuwait) containing recalcitrant petroleum hydrocarbons. The obtained microbial consortium was able to grow on and degrade the remaining petroleum hydrocarbons which could not have been utilized by the indigenous microbes in the original Kuwait soil. The following microbial community analysis using 16S rRNA gene sequencing suggested that the enhanced degradation of the remaining recalcitrant petroleum hydrocarbons by the novel microbial consortium may have been attributed to the selected bacterial populations belonging to Bacillus, Burkholderia, Sphingobacterium, Lachnospiraceae, Prevotella, Haemophilus, Pseudomonas, and Neisseria

Keywords: Kuwait petroleum-contaminated soil, Bioremediation, Total petroleum hydrocarbons(TPH), Selective enrichment, Microbial community analysis

This Article

  • 2021; 26(4): 20-26

    Published on Aug 31, 2021

  • 10.7857/JSGE.2021.26.4.020
  • Received on Apr 6, 2021
  • Revised on Apr 13, 2021
  • Accepted on Aug 19, 2021

Correspondence to

  • Joonhong Park
  • School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Korea

  • E-mail: parkj@yonsei.ac.kr