- Biodegradation of fluorene and bioremediation study by Sphingobacterium sp. KM-02 isolated from PAHs-contaminated soil
- Nam, In-Hyun;Chon, Chul-Min;Kim, Jae-Gon;
- Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources(KIGAM);Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources(KIGAM);Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources(KIGAM);
- PAHs 오염토양에서 분리된 Sphingobacterium sp. KM-02를 이용한 Fluorene 분해 및 토양복원 연구
- 남인현;전철민;김재곤;
- 한국지질자원연구원 지구환경연구본부 지질재해연구실;한국지질자원연구원 지구환경연구본부 지질재해연구실;한국지질자원연구원 지구환경연구본부 지질재해연구실;
Abstract
The fluorene-degrading strain Sphingobacterium sp. KM-02 was isolated from PAHs-contaminated soil near a mineimpacted area by selective enrichment techniques. Fluorene added to the Sphingobacterium sp. KM-02 culture as sole carbon source was 78.4% removed within 120 h. A fluorene degradation pathway is tentatively proposed based on identification of the metabolic intermediates 9-fluorenone, 4-hydroxy-9-fluorenone, and 8-hydroxy-3,4-benzocoumarin. Further the ability of Sphingobacterium sp. KM-02 to bioremediate 100 mg/kg fluorene in soil matrix was examined by composting under laboratory conditions. Treatment of microcosm soil with the strain KM-02 for 20 days resulted in a 65.6% reduction in total amounts. These results demonstrate that Sphingobacterium sp. KM-02 could potentially be used in the bioremediation of fluorene from contaminated soil.
Keywords: Fluorene;Sphingobacterium sp. KM-02;Metabolic intermediates;Microcosm test;fluorene contaminated soil;
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