• Effects of Heavy Metals on Biomonitoring using Recombinant Bioluminescence Bacteria
  • Kong, In Chul;Kim, Jin Young;Ko, Kyung-Seok;
  • Department of Environmental Engineering, Yeungnam University;Department of Environmental Engineering, Yeungnam University;Geologic Environment Division, Korea Institute of Geoscience & Mineral Resources;
  • 유전자재조합균주를 이용한 생물모니터링에 중금속 오염물이 미치는 영향 평가
  • 공인철;김진영;고경석;
  • 영남대학교 환경공학과;영남대학교 환경공학과;한국지질자원연구원;
Abstract
This research focused on the effects of heavy metals on the biomonitoring activity of genetically engineered bioluminescent bacteria, Pseudomonas putida mt-2 KG1206. KG1206 was exposed to single or binary mixtures of different heavy metals as well as soils contaminated with heavy metals. In case of single exposure with different inducer pollutant, the toxicity order was as followings : As(III) > Cd, As(V) >> Cu, Cr(VI). The toxic effects of the binary mixtures was compared to the expected effect based on a simple theory of probabilities. The interactive effects were mostly additive, while in few cases antgonistic and synergistic mode of action was observed for some concentration combinations. No considerable correlation was found between the total metal contents in soils and the bioluminescence activity of each sample. However, statistically significant differences (p = 0.0102) were observed between two groups, classified based on arsenite contamination. These results demonstrate the usage of recombinant bioluminescent microorganism in biomonitoring and the complex interactive effects of metals.

Keywords: Genetically engineered microorganism (GEM);Bioluminescence;Toluene analogs;Heavy metal;

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This Article

  • 2013; 18(4): 32-39

    Published on Aug 31, 2013

  • 10.7857/JSGE.2013.18.4.032
  • Received on May 8, 2013
  • Revised on Jun 18, 2013
  • Accepted on Jun 20, 2013