• Basic Study on Conditions and Analytical Methods of Biofilm Formation for the Bioassessment of Artificial Groundwater Recharge System
  • Kong, In Chul;Lee, So Ra;Ha, Kyoochul;Ko, Kyung-Seok;
  • Department of Environmental Engineering, Yeungnam University;Department of Environmental Engineering, Yeungnam University;Groundwater Department, Korea Institute of Geoscience and Mineral Resources;Groundwater Department, Korea Institute of Geoscience and Mineral Resources;
  • 지하수 인공함양시스템의 생물학적 평가를 위한 생물막 형성 조건 및 형성도 분석 방법에 관한 기초연구
  • 공인철;이소라;하규철;고경석;
  • 영남대학교 환경공학과;영남대학교 환경공학과;한국지질자원연구원 지하수연구실;한국지질자원연구원 지하수연구실;
Abstract
For the preliminary investigations of the bioclogging on groundwater artificial recharge system, studies for conditions and analytical methods of biofilm formation on sediments were performed. Based on the tested results, following conditions were determined for biofilm formation on batch process: optimum period for biofilm formation (30 days), the proper inoculating water (pond water), medium (minimum salt medium with 0.1% yeast extract). Procedures for the measurement of ATP and DHA were also determined. Biomass extract was used for ATP measurement, while sediment itself for DHA. Effects of metals on the biofilm formation were investigated under the determined conditions. Different sensitivities and orders were found depending on tested metals and measurement methods. In general, biomass measurement by ATP and viable cell count showed higher sensitivity than that of DHA. Following toxicity orders were also appeared for ATP and viable cell: Cu ≈ Cd > As(III).

Keywords: Bioclogging;Artificial recharge system;Biofilm;ATP;DHA;

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This Article

  • 2015; 20(4): 22-30

    Published on Aug 31, 2015

  • 10.7857/JSGE.2015.20.4.022
  • Received on Mar 13, 2015
  • Revised on Apr 28, 2015
  • Accepted on Jun 4, 2015